Jackson Immunoresearch
AffiniPure Goat Anti-Mouse IgG, Light Chain Specific
115-005-174
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We study virus-host interactions using a mouse model of infection. Here we are examining total IgG concentrations in the serum of mice following infection with a chronic virus.
ELISA
Mouse serum
10ug/mL - 4C overnight
PBS with BSA(3%) and tween(0.05%)
100uL mouse serum diluted 1:30,000, or standards - RT for 1-2 hours
100uL detection antibody diluted 1:5000 - 1hour at RT
TMB substrate
Sensitive antibody for use in ELISAs. We are able to detect significant changes in serum IgG concentrations.
N/A
Great antibody
Works great if used as recommended.