Very Reliable GL7 Antigen Antibody

March 10, 2017

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Catalog Number:

144604

Here we were examining germinal center B cell populations in the spleen following viral infection. We use this antibody to distinguish germinal center B cells from other B cell subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400 - 25 minutes on ice

Blocking Agent

FcR blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

This is one of our most commonly used antibodies. It has always been very reliable. Used in combination with B220 and CD95 markers, can easily distinguish germinal center B cells from other B cell subsets.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable

The Bad

N/A

The Bottom Line

Anybody wishing to quantify or isolate germinal center B cells should use this antibody.

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