Biolegend
Alexa Fluor® 488 anti-mouse CD4 Antibody
100423
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Browse Similar Products: CD4 Flow Cytometry Antibodies
Successfully detects CD4+ T cells in thymus, spleen, and lymph node with high signal.
Flow Cytometry
Mouse splenocytes
1:100 dilution on ice for 20-30 min
PBS + 2% FBS + 1 mM EDTA
None
Flow cytometry blue laser
Successfully detects mouse CD4+ T cells in splenocytes with good signal. There was a small population that the antibody partially binds to, more so than other fluorophore-conjugated mouse CD4+ antibodies. Additionally, some signal is lost during fixation
N/A
Detects CD4+ T cells with good signal
Partial binding to cells
Good antibody to detect CD4+ T cells by flow cytometry.