ALDH1A3 Staining In Dedifferentiated Pancreas Tissue

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

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Company:

Novus Biologicals

Product Name:

Aldh1A3

Catalog Number:

NBP215339

Our lab focused on loss of beta cell identity. We aimed to determine whether the dedifferentiation marker Aldh1a3 is expressed in the our mouse model in which several beta cells have lost identity.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreas cryosection

Primary Incubation

1:500, Overnight, 4 Deg Celcius

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS

Secondary Incubation

1:1000, Donkey anti-Rabbit Cy3

Tertiary Incubation

N/A

Detection

Immunofluorescence

Results Summary

Expression of Aldh1A3 found in both insulin+ and insulin-negative cells of our mouse model that loses beta cell identity.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Excellent detection at 1:500

The Bad

N/A

The Bottom Line

Excellent product

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