Great Phospho-Histone H3 (Ser10) Marker

March 13, 2017

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

Catalog Number:

06-570

We utilized this widely published rabbit polyclonal antibody to assess levels the amount of mitotic protein in a synchronized cell population. We were happy with its ability to produce a clean band and would use again. 

Experimental Design and Results Summary

Applications

Western Blot

Sample

U2OS cell lysate

Primary Incubation

1:500 at room temperature for 4 hours

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:5000 at room temperature for 1 hour

Tertiary Incubation

None

Detection

SuperSignal West Femto Maximum Sensitivity Substrate

Results Summary

U2OS cells were lysed and blotted for phospho-histone (ser10) H3 expression.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear binding of antibody.

The Bad

None. Clean.

The Bottom Line

Widely published and validated. Great antibody.

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