Great ECL Signal Kit

Albert Einstein College of Medicine
Physiology and Biophysics
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

SuperSignal West Femto Maximum Sensitivity Substrate

Catalog Number:

34095

This product is designed to enhance chemiluminescent substrate for horseradish peroxidase and detect even femtograms of protein on a Western Blot membrane. The very bright signal is excellent and highly recommended over other ECL kits.

Experimental Design and Results Summary

Application

Western Blots

Starting Material

Rat proteins bound to PVDF

Protocol Overview

Simply mix the two provided solutions in a 1:1 ratio and apply to blot. Wait 5 minutes, wash off and visualize

Tips

Only expose for brief periods as the signal is intense.

Results Summary

Sharp, intense bands with limited background signal.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

You can see proteins of low abundance.

The Bad

Exposure for too long can lead to signal burn.

The Bottom Line

Excellent for low abundance proteins but too strong for high abundance proteins.

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