MyD88 Antibody

March 10, 2017

Surgery
University of Pittsburgh
Research Scientist

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Company:

eBioscience

Product Name:

MyD88 antibody

Catalog Number:

14-6223-81

To test the stability of protein targets implicated in serum starvation, Hep G2 cells were used to perform the pulse-chase experiments by Western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hep G2

Primary Incubation

1/1000

Blocking Agent

5% milk

Secondary Incubation

1/1000

Tertiary Incubation

1 hour room temperature

Detection

Enhanced chemiluminescence kit

Results Summary

The expression of MyD was not changed after cycloheximide treatment

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The system worked

The Bad

The size of MyD was not as expected

The Bottom Line

The system worked

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