Detection Of Cell Viability

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

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Company:

ThermoFisher Scientific

Product Name:

Calcein Blue, AM

Catalog Number:

C1429

The aim of this experiment was to sort cells based on the fluorescent reporter. The viability of cells for sorting was detected using calcein blue. The calcein blue was recommended by colleagues.

Experimental Design and Results Summary

Application

For sorting cells (FACS)

Starting Material

Mouse Cells

Protocol Overview

1 ul of calcein blue was added per 1 ml of cells in flow buffer.

Tips

Cover from light.

Results Summary

Viability was detected using the sorter.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very stable.

The Bad

Light sensitive.

The Bottom Line

Calcein blue stains are very stable for the determination of cellular viability.

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