Corning
Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, *LDEV-Free, 10mL
356231
The aim of this experiment was to embed sorted thyroid progenitor cells in the basement membrane for differentiation and maturation into thyrocytes. The matrix was recommended by colleagues and is widely used in the literature.
Differentiation
Sorted mouse thyroid progenitor cells
Aliquots of matrigel were thawed on ice. Cells were pelleted and then mixed with 20 ul of media. Matrigel was added and mixed with the cells. Matrigel mixture was pipette as droplets in 12 well plate. The plate was incubated for 15-20 minutes and then media was added to the solidified drop.
Aliquot the matrigel for use to avoid repeated thawing which may introduce bubbles in the gel.
Thyrocytes were well formed and matured in the matrigel matrix.
N/A
Easy to use.
Expensive to purchase.
Matrigel is a standard basement membrane matrix used in differentiation protocols.