The Antibody Identifies CD25+ T-Cells Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 650™ anti-mouse CD25 Antibody

Catalog Number:

102038

We wanted to examine how T-cell populations shift in healthy vs diseased states. To do this, we wanted to examine the expression of CD25 on T-cells, so we used this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse lymph node cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody clearly identifies CD25+ T-cells very well

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD25+ cells very well

The Bad

None to mention

The Bottom Line

The antibody is good for looking at CD25+ T-cells

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