Anti Human CD4 Antibody For FACS

March 13, 2017

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Pharmingen™ PE Mouse Anti-Human CD4

Catalog Number:

555349

Our lab works on primary cell model of HIV latency. To do this we purify CD4 T cells and drive them into latency. In order to validate the purity of CD4 T cells after purification we stain this antibody to detect CD4+ cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Purified CD4 T cells

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good staining, minimal background

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Would recommend this antibody

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