Human CD45RO Antibody PE

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human CD45RO Antibody

Catalog Number:

304206

We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We use purified CD8 T cells from naive donors. We use different modalities to activate these cells and stain with CD45RO antibodies to detect cells that have differentiated to a memory T cell phenotype.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Transduced human CD8 T cells

Primary Incubation

1:100, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good clean staining for CD45RO

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good staining, minimal optimization required

The Bad

None

The Bottom Line

Would recommend

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