BD Biosciences
Propidium Iodide
556463
Browse Similar Products: Propidium Iodide Flow Cytometry Antibodies
This reagent was used to characterize living versus dead cells. It was chosen because it is the gold standard for measuring necrotic cells, PI is bright and works well with other fluorophores in the staining panel, and BD had a good price.
Flow Cytometry
Human T cells
5uL per test in 100uL total staining volume for 15 minutes at room temperature
2% FBS
N/A
PI staining was as expected, with dead cells clearly resolved from healthy living cells.
Works well, low cost.
Will continue to use this reagent.