Staining T Cells With Annexin V APC

February 24, 2017

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Annexin V APC

Catalog Number:

640920

This reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other fluorophores in the staining panel, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T cells

Primary Incubation

5uL per test in 100uL total staining volume for 15 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Annexin V staining was as expected, with apoptotic cells clearly resolved from healthy live cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well

The Bad

N/A

The Bottom Line

Will continue to use this reagent.

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