Staining Mouse MSC With Anti-CD90 PE

February 22, 2017

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

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Company:

Abcam

Product Name:

anti-mouse CD90 PE

Catalog Number:

ab24904

This antibody was used to phenotype mouse bone marrow derived mesenchymal stem/stromal cells. This antibody was chosen because it is a commonly used clone for CD90, and PE is bright and works well with other fluorophores in the staining panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse MSC

Primary Incubation

1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

All cells were positive for CD90, as expected. Positive cells were strongly stained compared to unstained cells, and would be fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

High cost

The Bottom Line

Will continue to use this antibody.

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