Anti-human CD45RA Antibody Review

February 17, 2017

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-human CD45RA Antibody

Catalog Number:

304126

Our lab has developed an in vivo humanized mouse model consisting of injecting human PBMC into NSG (immunocompromised) mice to study HIV pathogenesis and test therapeutic agents. Studies investigating different latency reversing agents and treatments involve a detailed analysis of different cell populations in the spleen including memory and effector CD8 and CD4 T cells. Antibodies distinguishing these populations are needed for this purpose.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC gated on CD4 T cells

Primary Incubation

1:100 for 30 min at 4 degrees Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good Staining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Work well

The Bad

None

The Bottom Line

I recommend this product

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