Use Of The Lynx Stereo Dynascopic Microscope From Vision Engineering For Passaging Human Pluripotent Stem Cells Cultures

February 16, 2017

BWH
Pathology
Research Scientist

Overall

Performance

Ease-of-Optimization

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Company:

Vision Engineering

Product Name:

Lynx Stereo Dynascopic Microscope

Catalog Number:

Lynx Stereo

This eyepiece-less system offers a convenient way to passage standard hiPS cell culture while under a biosafety cabinet. Optics allowed to visualize cell colonies and process them by cutting technics for culture routine maintenance in sterile conditions.

Experimental Design and Results Summary

Application

hiPSC sterile culture passaging

Starting Material

Human pluripotent stem cells (hiPSCs)

Protocol Overview

The Lynx is placed under a biosafety cabinet. Culture dish/plates are placed on the stage and while visualizing the cultures through the optic display, the user process individual colonies by the cutting method for passaging hiPSCs.

Tips

Adjust stage light and focus distance for optimal hiPSC colonies visualization

Results Summary

hiPSC cultures were routinely passaged under sterile environment using the Lynx that can fit standard biosafety cabinet. It also allowed to scrap undesirable colonies from the culture vessel.

Features Summary

Biosafety cabinet compatible, ergonomic

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Convenient system for sterile mechanical passaging/picking cell colonies

The Bad

Optimal contrasting of cell cultures can be challenging to achieve

The Bottom Line

A good system for mechanical passaging/picking cell colonies, in particular human pluripotent stem cells (hiPSCs). Fit biosafety cabinet to allow for sterile culture visualization and manipulation

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