Calmodulin Antibody

Health Sciences
Brock University
Postdoctoral fellow

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Company:

Novus Biologicals

Product Name:

Calmodulin

Catalog Number:

NB120-2860

In this test, we determined the efficacy of the mouse monoclonal calmodulin antibody for immunoprecipitation assays. Mouse soleus muscle was taken and 100 ug of protein was subjected to immunoprecipitation with 5 ug of calmodulin antibody. Western blotting for calmodulin reveals a clean band at the expected molecular weight of 17 kDa.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Mouse skeletal muscle

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Enhanced chemiluminescences

Results Summary

A clean band at 17 kda indicates successful immunoprecipitation of calmodulin. Experiments were run on 3 separate mouse soleus muscles.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The cleanliness

The Bad

Nothing

The Bottom Line

Awesome product

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