ProLong® Gold Antifade Mountant In Immunofluorescence Microscopy

The University of Toledo HSC
Medical Microbiology and Immunology
Graduate Research Assistant

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Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

ProLong® Gold Antifade Mountant with DAPI

Catalog Number:

P36931

I have used this mountant as the final step in preparing slides for imaging and it has worked very well. The DAPI stain is very clean and if stored properly, the slides are still good for imaging for long periods after staining.

Experimental Design and Results Summary

Application

Immnofluorescence microscopy

Starting Material

Mouse embryonic fibroblasts cells on LabTek slides

Protocol Overview

ThIs is a very good mounting medium to preserve stained slides and also imparts the cellular nuclei with a DAPI stain.

Tips

One tip is to use only an amount required to cover the slide at room temperature and keep the slides in the dark on a flat surface for some time before attempting to seal. This will ensure that the mount ant does not seep out and smear the slides.

Results Summary

This mountant provides good DAPI staining and keeps slides in good condition for a long time.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well

The Bad

Takes a long time thaw so must be prepared in advance

The Bottom Line

This is a good quality mountatnt and DAPI stain

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