Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 647

February 17, 2017

Medical Microbiology and Immunology
The University of Toledo HSC
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by ThermoFisher Scientific

Product Name:

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

Catalog Number:

A21245

This antibody was primarily applied in co-localization studies to stain proteins expressed ectopically in human cell lines.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Transfected and fixed HEK 293

Primary Incubation

1:1000 for 3 hours

Blocking Agent

BSA

Secondary Incubation

1:1000 for 1 hour

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

This antibody worked well in my studies and particularly looked good in cells with punctate morphology.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It was relatively easy to optimize

The Bad

Not particularly vibrant, but that is understandable due to the wavelength

The Bottom Line

I would recommend this antibody!

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