Goat Anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488

February 15, 2017

Medical Microbiology and Immunology
The University of Toledo HSC
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by ThermoFisher Scientific

Product Name:

Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Catalog Number:

A11029

This secondary antibody was used for immunofluorescence microscopy in both mouse and human cells. Overall, it works quite well and gives a clean staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Fixed mouse and human cells

Primary Incubation

1:2000 for 3 hours

Blocking Agent

BSA

Secondary Incubation

1:1000 for 1 hour

Tertiary Incubation

N/A

Detection

Confocal microscopy

Results Summary

With each use, I am able to achieve consistently vibrant staining with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to optimize, images well.

The Bad

Incubation must be strictly timed!

The Bottom Line

Highly recommend!

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