Works Well For Detection Of Gal-1 Over A Broad Range Of Concentration

February 10, 2017

Weill Cornell Medicine
Medicine
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bosterbio

Product Name:

Human Galectin-1 PicoKine™ ELISA Kit

Catalog Number:

EK0762

Human ovarian cancer ascites samples were tested for presence of Galectin-1 protein. Depending on the donor, ascites samples were found to have varying concentrations of Gal-1.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human Ovarian Cancer ascites

Protocol Overview

Cell-free ovarian cancer ascites samples were tested at a number of dilutions. 1:10 dilution was found to give consistent results.

Tips

If using for the first time, plate samples at at least 3-4 dilutions to get consistent results among samples at the same dilution.

Results Summary

Depending on the donor, human ovarian cancer ascites were found to have varying concentrations of Gal-1.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use.

The Bad

None.

The Bottom Line

Straightforward and easy to use once a general idea of Gal-1 concentration in sample of interest is optimized.

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