Thermo Fisher Scientific
Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase
K1671
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Our lab was looking for cheaper and easier alternative to the SuperScript RT kits. The Maxima kit works out cheaper and everything is supplied as one master mix so it is easier to set up a lot of reactions. Additionally, the entire RT process is completed in 30 minutes, which is much faster than our SuperScript III kit. I've used this RNA from a number of mouse cells to make cDNA prior to qPCR.
cDNA synthesis
Mouse RNA
Add 200ng RNA, DNase, and DNase buffer and put at 37° for 2 min. Add RT enzyme and buffer and put at 25° for 10 min followed by 50° for 15 min. Dilute cDNA and it is ready to use
Do not exceed the recommend amount of RNA
Works well for concentrations around 200ng, I have had mixed results once I use around 1ug RNA.
N/A
Fast, easy
I've had mixed results around 1ug+ of RNA
Great kit, everything is in an easy mastermix, entire process is super fast.
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