Anti-CD-62P-AF647 Ab For The Detection Of Activated Human Platelets By Flow Cytometry

February 15, 2017

Clinical Research
Albert Einstein College of Medicine
Pre-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Mouse anti-human CD-62P-Alexa Fluor 647

Catalog Number:

NB100-65391AF647

We use Novus Biological's Anti-CD-62P-AF647 Ab in concert with an anti-human CD41a Ab (BD) to detect activated human platelets by flow cytometry. The two-color assay is performed on fresh platelets and is very simple once the entire protocol has been optimized. Together, these antibodies provide us with clean, consistent, and easy to interpret data.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Platelets

Primary Incubation

Incubate fresh platelets (1x10^6) with molecules of interest for 20 minutes at room temperature.

Blocking Agent

Not applicable.

Secondary Incubation

Add 10 ul of anti-CD62P Ab and incubate for 20 minutes on ice.

Tertiary Incubation

No tertiary antibody/incubation

Detection

Positive staining can be detected using a flow cytometer (we use an LSR-II).

Results Summary

Following data acquisition, analyses can be performed using flow cytometry software such as FlowJo. Make sure to run single color and FMO controls with each experiment to ensure the utmost accuracy of results.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Simple protocol, easy to optimize, reliable & consistent results.

The Bad

Slightly expensive. Make sure to titrate/optimize antibody with your sample type prior to use on precious samples/materials.

The Bottom Line

In combination with an anti-human-CD41a Ab, I highly recommend this product for flow cytometry-based detection and quantification of (activated) human platelets. As stated, the combined two-color staining is very easy to read/interpret, and the overall protocol is stress-free and time efficient.

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