B220 Staining Of Spleen Section

February 09, 2017

Immunology
Northwestern University
Grad Student

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Company:

BD Biosciences

Product Name:

Biotin Rat Anti-Mouse CD45R/B220

Catalog Number:

553086

Mice were immunized and spleens were removed and sectioned. Spleens were then stained with anti-B220 for immunohistochemistry staining.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse Spleen

Primary Incubation

1:100, overnight, 4 degrees

Blocking Agent

FBS

Secondary Incubation

None

Tertiary Incubation

SA-HRP, 1:250, 30 min, room temperature

Detection

AEC substrate

Results Summary

Clear staining of B cells in the spleen

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody for B cells staining in immunohistochemistry

The Bad

None

The Bottom Line

Good antibody for B cell staining in the spleen.

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