Developmental Studies Hybridoma Bank
EC11 anti-Pericardin Antibody
EC11 anti-Pericardin-S
Browse Similar Products: prc Immunofluorescence Antibodies
We study extracellular matrix in the Drosophila melanogaster heart. Pericardin is one such matrix protein in the fly heart which can change in expression with age. We needed an antibody to not only stain the pericardin protein in order to visualize, but also to quantify relative protein changes with age. We selected this product since it was the only available product out there, and it worked great once we optimized staining protocol.
Immunofluorescence
Drosophila melanogast heart
1:40 anti-pericardin in .1% Triton- X in PBS [PBST], overnight, 4 degrees
1% Seablock in PBS for 1 hour
1:1000, 1 hour, room temp in .1% PBST of Donkey Anti-Rabbit alexa 647
None
LSM 710 Zeiss microscope in far red channel
Great Results. Beautiful staining. Bright and easily quantifiable between samples. Pericardin is a protein with domains closely homologous to collagen IV, but this antibody only stains Pericardin and not the Drosophila Collagen IV protein [Viking] which is entirely different. Highly recommend this antibody for use in staining Pericardin in Drosophila melanogaster.
10.1016/j.matbio.2016.10.008
N/A
Great Pericardin Antibody
Needs high dilution concentration. Probably needs a little adjusting lab to lab.
Highly recommend.