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Biotin anti-mouse CD8a Antibody
100704
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In our lab we work with different lentiviral vectors that we use to transduced primary human cells. some of these constructs utilize a truncated mouse CD8 protein. We use the biotynilated mCD8 antibody in combination with streptavidin magnetic beads to purify cells that have been successfully transduced with the lentiviral vectors.
Affinity Purification
Transduced human CD8 T cells
1:100, 15min, room temperature
None
Streptavidin beads, 10min, room temperature
We detect purity of the mCD8+ cell fraction by flow cytometry after staining with mCD8a antibody
By using this antibody for affinity purification we consistently get a highly pure population of mCD8a positive
N/A
Good purity of mCD8 positive cells
Good biotynilated antibody to use to affinity purification of mouse CD8 positive cells