CD184 (Cxcr4) Detection By Flow Cytometry

Center for Regenerative Medicine
Boston University
Postdoctoral Associate

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Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen

Product Name:

APC Rat anti-Mouse CD184

Catalog Number:

558644

The aim of this experiment was to detect the expression of CD185 on differentiated mouse embryonic stem cells. The overall objective of this research was to detect endoderm generation on Day 5 of the mouse directed differentiation protocol using flow cytometry detection. This product has been extensively used in the department, and recommended by colleagues. In addition, there is wide literature available on it.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse embryonic cells

Primary Incubation

5 ul of antibody in 100 ul of sample. 45 minutes incubation period over ice.

Blocking Agent

Not applicable

Secondary Incubation

Already conjugated so no secondary incubation

Tertiary Incubation

Not applicable.

Detection

Flow cytometry.

Results Summary

The distinct expression of CD184 was detected using flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reproducible antibody.

The Bad

Price

The Bottom Line

Both reliable and reproducible antibody.

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