Circulating Immune Complex (CIC) Detection And Quantification Using Quidel's MicroVue Enzyme Immunoassay Technology

January 26, 2017

Albert Einstein College of Medicine
Clinical Research
Pre-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Quidel

Product Name:

MicroVue CIC-C1q enzyme immunoassay (EIA)

Catalog Number:

A001

The MicroVue CIC EIA kit can be used to quantify CICs in small volumes (<10 ul) of human serum or plasma. Each kit includes one 96-well plate that has been coated with purified, human C1q protein. Complement fixing (active) CIC bind to C1q, allowing for easy quantification using the manufacturer's protocol. The kit includes all reagents required for completion of the assay which takes approximately 2 hours from start to read time. A plate reader (405 nm) is required for quantification of sample CIC concentrations.

Experimental Design and Results Summary

Application

Circulating immune complex quantification by EIA

Starting Material

Human serum or plasma

Protocol Overview

The wells of the C1q-coated plate must be rehydrated prior to sample application (reagent included). Samples are then applied at the proper dilution for 1 hour at room temperature followed by wash steps. Conjugate is added to each well (followed by wash steps), followed by the application of the substrate solution and then the stop solution according to the product manual's guidelines. The OD values must be obtained within 1 hour of assay completion.

Tips

Plate/dilute your samples in a separate, uncoated plate to allow for uniform plating via a multi-channel to the CIC plate. Plate positive and negative controls (not included) on each plate to ensure the utmost control of experimental quality.

Results Summary

OD values must first be obtained from a standard plate reader (405 nm). A standard curve can then be generated from the three standards included in the kit (A, B, and C). The standard curve must meet the quality control requirements outlined in the product manual prior to calculating the experiment sample CIC concentrations.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Each kit includes multiple vials of each reagent used for the plate. Time efficient (~2 hours from start to finish). The plate is made up of 12 individual colums of 8 wells allowing for unused wells to be saved for a second or third run.

The Bad

Kits are relatively expensive.

The Bottom Line

I would recommend this kit to anyone looking for a simple, quick method for the quantification of CICs in human samples. The protocol is very easy to follow, and 1 kit goes a long way with the amount of reagent provided and the ability to save/store unused wells for future use.

Join the discussion