Radial Immunodiffusion Plates To Measure Human IgG Subtypes (Including IgG1/2/3/4) In Serum, Plasma, Or Other Biological Fluids

January 24, 2017

Albert Einstein College of Medicine
Clinical Research
Pre-doctoral researcher

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Company:

The Binding Site Ltd.

Product Name:

IgG subtype (IgG 1/2/3/4) Radial Immunodiffusion Kits

Catalog Number:

RN106.3/RN107.3/RN108.3/RN109.3

The IgG subtype RID kits from The Binding Site, ltd. are a simple, reliable benchtop assay for the quantification of individual IgG subtypes in human serum or plasma. The company makes individual kits for each subtype (i.e. one kit of 3 IgG1 RID plates), and the experimental protocol is consistent across all subtypes making the RID plates even easier to use. We utilize the kits to quantify IgG subtype quantities in all of our pediatric (human) plasma samples and have yet to be disappointed with the product and the quality/reliability of our results.

Experimental Design and Results Summary

Application

Human Immunoglobulin G subtype (IgG1/2/3/4) Quantification

Starting Material

Human serum, plasma, biological fluid

Protocol Overview

Three standards (included), a control sample (included), and the experimental samples are applied to individual wells on the RID plate according to the manufacturer's suggestions (volumes and dilutions are suggested depending on the sample population). Once filled, each RID plate should be closed and stored at room temperature for a minimum of 72 hours prior to reading the diffusion diameters.

Tips

Allow each plate to warm to room temperature prior to sample application. Wipe off/remove any condensation from the RID plate prior to plating the samples/standards/controls, as the condensation will result in diluted samples and ultimately error. Research the 'normal range' for your sample/patient population -- dilution recommendations are provided by the manufacturer but do not apply to all sample types.

Results Summary

The IgG subtype plates can be read in as little as 72 hours (minimum incubation time). The diameters of the 3 standards and the control sample should be read first using a sub-mm ruler under magnification or an RID plate reader (if available). A standard curve can be generated using the diameter^2 of each standard of known concentration. The user's manual provides quality control measures for both the standards and the control sample. The experimental sample diameters should then be determined, squared, and entered into the standard curve equation for determination of each sample's IgG subtype (i.e. IgG1) concentration.

DOI or PMID #

N/A

Additional Notes

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Summary

The Good

High quality results, low stress, time efficient, reliable company.

The Bad

Relatively expensive, not sold by many outside retailers -- must contact company for quote

The Bottom Line

I highly recommend all four IgG subtype RID kits to anyone who is willing and able to pay a little bit more for a simple, reliable assay. Samples can be plated within minutes, and results are available in as little as 72 hours (the minimum incubation time). All reagents required for the assay, as well as a detailed user's manual, are included in the kit, making the RID technology incredibly simple to use.

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