Isotype Control for K9 Immunophenotyping

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

Biolegend

Product Name:

APC Rat IgG1, λ Isotype Ctrl

Catalog Number:

401903

The aim of this experiment is to detect presence of T or B cell lymphoma in dogs. This isotope control was used to mark the negative population of cells aka the cells that did not or negatively stained in the APC channel. This antibody served the purpose very well as acting as a negative control.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Dog whole blood in EDTA

Primary Incubation

30 mins in the dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Serves as a good isotype control.

DOI or PMID #

NA

Additional Notes

NA

Image Gallery

Summary

The Good

Serves as a good isotype

The Bad

None

The Bottom Line

Will recommend to others.

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