Anti-IL10 Antibody

February 02, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

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Company:

Abcam

Product Name:

Anti-IL10 antibody [JES5-2A5]

Catalog Number:

ab33471

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue homogenates

Primary Incubation

1:800 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:2500 at room temperature for 2 hour

Tertiary Incubation

NA

Detection

Western Blotting

Results Summary

IL-10 proteins were detected in mice tissue homogenates.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal with primary antibody

The Bad

Gives little background

The Bottom Line

Good antibody for mouse IL-10 detection

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