Detects FLT3 Expression In Human Leukemic Cells

January 25, 2017

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Human CD135 (Flt3) Antibody PE BV10A4H2 RUO

Catalog Number:

12-1357

The general aim of my research is to study cytokine receptor expression and signaling in human leukemic cells. We are measuring cytokine receptor expression in human leukemia cell lines.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP1 leukemic cell line from human blood

Primary Incubation

1:50

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

This antibody consistently detects FLT3 expression in this cell line relative to an isotype control antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good detection of surface FLT3 expression

The Bad

None

The Bottom Line

Recommend this antibody for flow cytometry

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