Detects cKit Expression In Human Leukemic Cells

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

Anti-Human CD117 (c-Kit) PE

Catalog Number:

12-1178

The general aim of the research is to study cytokine receptor signaling in acute myeloid leukemia. This antibody was used to measure expression level of cKIT receptor in human AML cell lines.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

TF1 erythroleukemic cell line from human blood

Primary Incubation

1:50

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

Expression of cKIT was detected in all cells of the cell line compared to an isotype control antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

Recommend this product for flow cytometry

Share your experience with other scientists. Write a Review! »

Join the discussion