p38 MAPK (D13E1) XP® Rabbit mAb

January 30, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p38 MAPK (D13E1) XP® Rabbit mAb #8690

Catalog Number:

8690

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine ex-vivo immune cell assay lysates

Primary Incubation

1:1000 at 4 degrees for overnight incubation in 5% BSA

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:3000 at room temperature for 2 hour in 5% skimmed milk

Tertiary Incubation

None

Detection

Western Blotting

Results Summary

High levels of p38 proteins were detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

specific antibody for p38 MAPK

The Bad

None

The Bottom Line

Good antibody for p38 MAPK detection. works better with overnight incubation in 5% BSA solution.

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