Fast Easy RNA Clean Up

January 19, 2017

UCSF
Urology
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo

Product Name:

RNA Clean & Concentrator-5

Catalog Number:

R1015

These columns work well to clean up RNA and elute in a small volume.

Experimental Design and Results Summary

Application

Clean up RNA between steps when making sequencing libraries.

Starting Material

RNA

Protocol Overview

Resuspend RNA in binding buffer, flow through columns, centrifuge, wash and centrifuge, add elution buffer and elute into new tubes

Tips

Make sure to fully remove all wash buffer

Results Summary

Clean and concentrated RNA as long as you have enough starting RNA

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy, works well with sufficient RNA

The Bad

You lose a fair amount of RNA, with low inputs within the specifications of the kit you often still get some carryover of wash buffer and less clean eluted RNA.

The Bottom Line

Easy and good way to clean up RNA as long as you have enough material going into the kit.

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