Abcam (Ab32087) Anti-MCL-1 Antibody For Western Blotting Mouse MCL-1

Biochemistry and Molecular Biology
University of Arkansas for Medical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-MCL1 antibody [Y37]

Catalog Number:

ab32087

This antibody was used for the western blot detection of mouse MCL-1 in primary lymphocytes. These cells were grown in RPMI with 10% FBS. This antibody is very specific and no background was seen in whole cell lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocytes

Primary Incubation

1:1000 dilution. 16 hours at 4°C in 1% Milk PBSTween

Blocking Agent

5% milk

Secondary Incubation

Goat Anti-Rabbit IgG (Upstate) 1:3000 in 1% milk PBSTween

Tertiary Incubation

NA

Detection

ECL+

Results Summary

Produced a very clean blot with the predicted MCL-1 bands at ~38 and ~36 kDa.

DOI or PMID #

NA

Additional Notes

Very good, clean antibody

Related Categories

Image Gallery

Summary

The Good

Very specific

The Bad

Expensive

The Bottom Line

For a steep price you can get a very specific antibody for mouse MCL-1. For a comparable cheaper antibody check our Rockland's MCL-1 antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion