Abcam
Thrombin-Antithrombin Complexes ELISA Kit (TAT)
ab137994
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Aim of the present study was to test the effect of feeding milk samples on formation of Thrombin-Antithrombin Complexes in diabetic mice. The selection of abcam Thrombin-Antithrombin Complexes ELISA kit was based on its price and merits shown in kit flyer.
Quantification of Thrombin-Antithrombin Complexes ELISA mice blood plasma
Blood from mice in collection tube, Heparin, DPBS, Centrifuge, Pipette, ELISA reader, Abcam Thrombin-Antithrombin Complexes ELISA kit.
Ocular Blood was collected in a heparinized tube from mice of all groups and mixed gently. Tubes containing heparinized blood was centrifuged at 275 xg for 5 minutes and pale yellow supernatant (Plasma) was collected. Plasma was used for quantification of Thrombin-Antithrombin Complexes ELISA as per manufacture instructions. In brief, plasma was added to ELISA plate well and incubated for 2 hours at room temperature. After washing wells were washed five times with washing buffer and biotin conjugated anti- Thrombin-Antithrombin Complexes antibody was added and incubated for 1 hour at room temperature. Wells were washed four times and treated with streptavidin peroxidase conjugate. Finally, colour was developed by adding chromogen substrate and colour intensity was read under a microplate reader.
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Thrombin-Antithrombin Complexes were very easily detected with very high sensitivity
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Easy to use and great sensitivity
Excellent abcam Thrombin-Antithrombin Complexes ELISA kit
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