Mouse IFN gamma ELISA Kit

January 13, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Mouse IFN gamma ELISA Ready-SET-Go

Catalog Number:

88-7314-86

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

ELISA

Sample

Ex-vivo mouse T cell assay supernatant

Primary Incubation

Overnight incubation at 4 degree with capture antibody

Blocking Agent

Blocking with 200 µL/well of 1X ELISA/ELISPOT Diluent

Secondary Incubation

Overnight incubation assay supernatant at different dilutions

Tertiary Incubation

1 hour incubation with detection antibody at room temperature

Detection

ELISA-liquid TMB substrate

Results Summary

IFN-gamma release was observed by immune cells upon ex vivo stimulation with antigens.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Nice efficient IFN-gamma detection

The Bad

None

The Bottom Line

Nice efficient IFN-gamma detection with detection limit of 15 - 2000 pg/mL

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