Promega
CytoTox 96 NonRadioactive Assay
G1780
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This assay is super easy and can easily be coupled to other assays, as you only take the supernatant from the cells to look at released LDH. It is measured with a 30-minute coupled enzymatic assay, which results in the conversion of a tetrazolium salt (iodonitrotetrazolium violet; INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells which can be correlated to apoptosis/necrosis.
Cell Culture Assays
PC12 cells which are of rat origin, and primary mouse neurons
Supernatant is transfered to a new plate and the CytoTox reaction buffer is added and incubate for 30minutes. Add stop solution and measure absorbance at 490nm, using a plate reader
Works best if you are doing an experiment that doesn't require any media changes. Works best in white walled plates
The absorbance measurements are proportional to the LDH levels. Increased LDH levels indicate cell death. It helps to have both a positive and negative control here. It is easy to look at toxicity, but harder if you are looking to rescue toxicity because the toxic substance would need to be carefully titrated.
N/A
Easy and can be coupled to other assays
None!
If you are at all interested in cell death in your assay system give this a try. You only need supernatant so its easy to add on to another experiment.
Product Name: CytoTox 96 Non-Radioactive Cytotoxicity AssaySupplier Page