Dendrite Marker In Primary Mouse Neurons

January 27, 2017

Research
Aquinnah Pharmaceuticals
Scientist

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Company:

Abcam

Product Name:

Rabbit polyclonal to MAP2

Catalog Number:

ab32454

Used this antibody as a dendrite marker to measure dendrite length. However, this antibody stained very weakly and we were unable to use this for our analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Primary Mouse Neurons

Primary Incubation

1:1000 in 5% Donkey Serum at 4C overnight

Blocking Agent

10% Donkey Serum at RT 1hr

Secondary Incubation

1:400 Dylight Donkey anti-rabbit

Tertiary Incubation

None

Detection

Microscopy

Results Summary

Antibody only weakly stained processes and we were unable to use this for our analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

Weak signal

The Bottom Line

There are better antibodies to use as dendrite markers. Don't waste your time with this one.

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