EMD Millipore
anti-Tubulin, Clone: TU-20
MAB1637
Browse Similar Products: Tubulin Immunofluorescence Antibodies
Antibody stained dendrites well, but left background speckling.
Immunofluorescence
Primary Mouse Neurons
1:500 in 5% Donkey Serum at 4C overnight
10% Donkey Serum at RT 1hr
1:400 Dylight Donkey anti-mouse
None
Microscopy
Staining was good for marking dendrites, but fades as you move more distally. To quantify you must up the intensity to find distal processes, but this overexposes proximally. Staining also left background speckles that made automated quantification impossible, but okay for manual.
N/A
Stains dendrites
High background
Good antibody, recommended by others. Would work well as a marker visually, but difficult for analysis.