Invitrogen
beta-3-tubulin, monoclonal antibody, clone - TU-20
MA119187
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This dendrite marker stains very well, but leaves background speckles in primary neuron cultures.
Immunofluorescence
Primary Mouse Neurons
1:500 in 5% Donkey Serum at 4C overnight
10% Donkey Serum at RT 1hr
1:400 Dylight Donkey anti-mouse
None
Microscopy
Staining for dendrites left very strong staining, but high background with lots of speckling. This allowed for good manual tracing of dendrites, but difficult for automated tracing due to background. Perhaps it would be possible to titrate the concentration down.
N/A
Strong dendrite signal
High background
Works well if you are just looking to visualize dendrites or run manual analysis. High background prevented us from doing any automated analysis.