R&D Systems
Neuron-specific beta-III Tubulin Mouse anti-Multi-species, Clone: TuJ-1
MAB1195
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After testing many antibodies for dendrite markers, this one was middle of the pack. Did a decent job staining dendrites, but didn't mark distal processes as well and left some background speckling.
Immunofluorescence
Primary Rat and Mouse Neurons
1:200 in 5% Donkey Serum at 4C overnight
10% Donkey Serum at RT 1hr
1:400 Dylight Donkey anti-mouse
None
Microscopy
Antibody marked dendrites, but did not reach distal processes. There was also background speckling. This made our tracing and analysis difficult.
N/A
Decent marker for dendrites if you aren't tracing
Doesn't reach distal processes and background issues
There are better dendrite markers.