Santa Cruz Biotechnology, Inc.
eIF3eta (N-20) Antibody
sc-16377
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This antibody has always worked well for staining cells and tissues, showing diffuse cytoplasmic staining under normal conditions and punctate under stress/disease.
Immunofluorescence
PC12 cells
1:300 in 5% Donkey Serum at 4C overnight
10% Donkey Serum at RT 1hr
1:400 Dylight Donkey anti-goat
None
Microscopy
Antibody works well for staining in mouse, rat, and human cell lines, as well as mouse and human tissue. Shows diffuse staining and localization in the cytoplasm. Upon punctate presentation this indicates translational arrest and localization to stress granules.
N/A
Works well for staining
Good antibody as a translational staining marker in all cell lines and tissues tried.