Great Antibody for TIA-1

January 17, 2017

Research
Aquinnah Pharmaceuticals
Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

TIA-1 Antibody (C-20)

Catalog Number:

sc-1751

I have tested many TIA-1 antibodies to find one better than this... I have not found one. This antibody works well in human and mouse tissue, for IP, and OK for Western Blots. Although the antibody needs to be used at higher concentrations, there is not another I have found to stain human and mouse tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PC12 cells

Primary Incubation

1:100 in 5% Donkey Serum at 4C overnight

Blocking Agent

10% Donkey Serum at RT 1hr

Secondary Incubation

1:400 Dylight Donkey anti-goat

Tertiary Incubation

None

Detection

Microscopy

Results Summary

Antibody stains cells and tissues well, showing mostly nuclear staining and upon stress translocation to the cytoplasm and granule formation. Has worked for cell lines, human and mouse tissues, IPs, and at 1:500 via Western Blot but not great. The issue with this antibody is lot reproducibility, we have lots that work great and other lots that don't work at all.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Best antibody we have found for all applications

The Bad

Lot reproducibility not good

The Bottom Line

Worth a try for any application. I have found others that work better via Western blot, but none that work as well on human or mouse tissue.

Share your experience with other scientists. Write a Review! »

Join the discussion