Santa Cruz Biotechnology
ATF-1
sc-270
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ATF1 belongs to a activating transcription factor sub family and basic region leucine zipper family. It plays an important role in various cellular physiologic processes by regulating the expression of downstream target genes which are responsible for other cellular activities.Here we checked the time course effect of chemoattractant in macrophages.
Western Blot
Mouse cell lysate
1:500 in 5% BSA prepared in 1x TBST
5% milk prepared in 1x TBST
1:4000 prepared in 1x TBST
None
ECL solution
We found time dependent increase of ATF1 expression by chemoattractant.
N/A
No background and the signal is prominent.
Very good for western blot. Highly recommended.