BD Biosciences
PE Rat Anti-Mouse CD4
553653
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The goal of this research was to clearly define the leukocyte populations present within a subcutaneous tumor-draining lymph noder.
Flow Cytometry
Mouse Lymph Node Leukocytes
Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice.
Fc block (TruStain FcX)
Wash cells once in FACS buffer. Stain with isotype control or CD4 antibody (1:500 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL PBS containing 1.5% paraformaldehyde.
N/A
Flow Cytometer
CD4+ T lymphocytes were clearly distinguished (middle panel, shaded histogram in right panel) from background (left panel, empty histogram in right panel).
Clearly labels CD4+ T cells.
No complaints.
Bright option for identifying CD4+ T cells.