EpCAM Antibody For FACS

January 05, 2017

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse CD326 (Ep-CAM) Antibody

Catalog Number:

118216

We are interested in studying crosstalk between murine colonic cell types and immune cells. We used the EpCAM antibody as a marker for the epithelial compartment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

EpCAM positive cells are clearly distinguished with this antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very clear staining with no background problem

The Bad

None

The Bottom Line

Highly recommended!

Share your experience with other scientists. Write a Review! »

Join the discussion