Good phospho-NF-kB p65 Antibody

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-NF-κB p65 (Ser536) Antibody

Catalog Number:

3031S

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse whole cell lysates

Primary Incubation

1:1000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:3000 at room temperature for 2 hour in 5% skimmed milk

Tertiary Incubation

NA

Detection

Western Blot

Results Summary

NF-kB p65 induction was observed with antigen stimulation.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good signal with primary antibody

The Bad

None

The Bottom Line

Good phospho-NF-κB p65 antibody that detects Ser536 phosphorylation.

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